ELIXIR CZ is entering a new operational stage, facing challenges from growing user numbers and emerging life science technologies. The challenge has evolved from data overflow to a multifaceted problem, presenting a significant chance for versatile solutions that are compatible, resilient, and user-focused. Being part of the European infrastructure ELIXIR, ELIXIR CZ will leverage its recognized expertise. A clear strategy is vital to uphold dedication, compatibility with European partners, and global relevance. In line with the ELIXIR CZ Scientific Advisory Board’s assessment, we developed a strategy based on our strengths and expertise, with five recommended growth areas. This forms the foundation for the ELIXIR CZ Strategy for years 2020-2025.